HTGAA: 1536

Only approved contributors can edit cell-free reaction compositions.
Cell-Free Reaction Compositions
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3. Adjust reagents
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Reagent
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Cell Lysate
1X
6.000 uL
-
DNA Template
50nM
2.000 uL
-
Nuclease-Free Water
N/A
2.000 uL
-
Potassium Glutamate
0.875M
312.563 mM
-
Magnesium Glutamate
0.5M
6.975 mM
-
HEPES-KOH pH 7.5
1M
45.000 mM
-
17 Amino Acid Mix
0.05M
4.063 mM
-
Tyrosine pH 12
0.05M
4.063 mM
-
Cysteine
0.2M
4.000 mM
-
Ribose
100g/L
11.625 g/L
-
AMP
0.1M
0.625 mM
-
CMP
0.1M
0.375 mM
-
GMP
0.1M
-
-
UMP
0.1M
0.375 mM
-
Guanine
0.025M
0.156 mM
-
Glucose
200g/L
1.250 g/L
-
Potassium phosphate dibasic
0.5M
5.625 mM
-
Potassium phosphate monobasic
0.5M
5.625 mM
-
Nicotinamide
0.1M
3.125 mM
-
Reagent Supplement JSON
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Summary A real-time, global, collaborative fluorescent protein artwork canvas designed by the How To Grow (Almost) Anything (HTGAA) community.
HTGAA students and TAs can place one pixel at a time, publish it to the shared artwork, then wait 20 seconds before placing another.
The artwork will be used in a cell-free protein synthesis optimization experiment designed by HTGAA students.
Experimental details: 2026 HTGAA Cloud Lab Recitation
Inspiration credit: r/place
Artwork Contributors 0 contributors · 0 contributions
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