DNA Gel Art Interface

Gallery pictures1
15 kb
10 kb
8 kb
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2 kb
1 kb
850 bp
650 bp
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100 bp

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15 kb
10 kb
8 kb
7 kb
6 kb
5 kb
4 kb
3 kb
2 kb
2 kb
1 kb
850 bp
650 bp
500 bp
400 bp
300 bp
200 bp
100 bp

Restriction Enzymes

Restriction Digests

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Water

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CutSmart Buffer

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λ DNA

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Enzyme(s)

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Restriction Digest Parameters

37°C for 60 minutes (incubation)
80°C for 20 minutes (heat inactivation) (optional)

DNA Gel Electrophoresis

Goal: 100-150 ng of DNA per lane!
Hand-Cast Gel
Digest
14.7 μL Water
3.3 μL Loading Dye
2 μL Digest
Ladder
6.6 μL Water
3.3 μL Loading Dye
10 μL Ladder (15 ng/μL Stock)
E-Gel
Digest
18 μL Water
2 μL Digest
Ladder
10 μL Water
10 μL Ladder (15 ng/μL Stock)

Documentation

Take lots of pictures and document both good & bad gels on your website!
Benchling
λ Phage DNA

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Note: designs are posted the 'All' tab until approved by an admin